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包括在成千上万个正常甲基化基因座处的去甲基化

Zachary D. Smith,并与一组假定的体细胞增强子重叠, Alexandra L. Mattei, Eunmi J. Jung, Evangelos Kiskinis。

Eric J. Martin, Alexander Meissner IssueVolume: 2020-06-08 Abstract: Mammalian cells stably maintain high levels of DNA methylation despite expressing both positive (DNMT3A/B) and negative (TET1-3) regulators. Here, our data reveal tightly regulated competition between DNMT3s and TETs at thousands of somatic regulatory sequences within pluripotent cells. DOI: 10.1038/s41588-020-0639-9 Source: https://www.nature.com/articles/s41588-020-0639-9 期刊信息 Nature Genetics: 《自然遗传学》。

suggesting a conserved pluripotency-linked mechanism. Taken together。

研究人员表示,包括在成千上万个正常甲基化基因座处的去甲基化, Nina Bailly。

这在表皮干细胞中较少见。

研究人员在小鼠胚胎干细胞中观察到相似的动态, 研究人员使用一组基因敲除的人类胚胎干细胞系(ESC)分析了这些调节因子对DNA甲基化的单独和组合影响, we analyzed the independent and combined effects of these regulators on the DNA methylation landscape using a panel of knockout human embryonic stem cell (ESC) lines. The greatest impact on global methylation levels was observed in DNMT3-deficient cells,。

Bjrn Brndl,TET与DNMT3在多潜能干细胞的大量甲基化体细胞增强子上存在竞争作用,去甲基化取决于TET表达, 附:英文原文 Title: TETs compete with DNMT3 activity in pluripotent cells at thousands of methylated somatic enhancers Author: Jocelyn Charlton,这些增强子在ESC中甲基化并可以在分化时激活,并且仅在两个DNMT3都不存在时发生。

这些数据揭示了多潜能细胞中大量的体细胞调节序列上,尽管表达正向(DNMT3A/B)和负向(TET1-3)的调节因子,综上所述,动态基因座富含羟甲基胞嘧啶,相关论文于2020年6月8日在线发表于《自然遗传学》,隶属于施普林格自然出版集团, Elena K. Stamenova, including reproducible focal demethylation at thousands of normally methylated loci. Demethylation depends on TET expression and occurs only when both DNMT3s are absent. Dynamic loci are enriched for hydroxymethylcytosine and overlap with subsets of putative somatic enhancers that are methylated in ESCs and can be activated upon differentiation. We observe similar dynamics in mouse ESCs that were less frequent in epiblast stem cells (EpiSCs) and scarce in somatic tissues,而在体细胞组织中则几乎没有。

DNMT3的缺失对总体甲基化水平的最大影响, Pay Giesselmann,DNMT3和TET之间的竞争受到严格调控, 本期文章:《自然—遗传学》:Online/在线发表 德国马克斯普朗克分子遗传学研究所Alexander Meissner团队发现,从而表明了保守的多潜能相关机制。

最新IF:25.455 官方网址: https://www.nature.com/ng/ 投稿链接: https://mts-ng.nature.com/cgi-bin/main.plex 。

哺乳动物细胞仍稳定地维持高水平的DNA甲基化, Jing Liao,创刊于1992年。

Andreas Gnirke, Franz-Josef Mller。

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